Molecular Detection of Potato Spindle Tuber Viroid in Razavi and Northern Khorasan Provinces
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چکیده
منابع مشابه
Insight on Genes Affecting Tuber Development in Potato upon Potato spindle tuber viroid (PSTVd) Infection.
Potato (Solanum tuberosum L) is a natural host of Potato spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion of leaves and tubers. PSTVd is the type species of the family Pospiviroidae, and can replicate in the nucleus and move systemically throughout the plant. It is not well understood how the viroid can affect ...
متن کاملGenetic variability of potato spindle tuber viroid RNA replicon.
The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected fo...
متن کاملPotato spindle tuber viroid: the simplicity paradox resolved?
TAXONOMY Potato spindle tuber viroid (PSTVd) is the type species of the genus Posipiviroid, family Pospiviroidae. An absence of hammerhead ribozymes and the presence of a 'central conserved region' distinguish PSTVd and related viroids from members of a second viroid family, the Avsunviroidae. PHYSICAL PROPERTIES Viroids are small, unencapsidated, circular, single-stranded RNA molecules which...
متن کاملogenicity, viroid evolution, Citrus exocortis viroid, Potato spindle tuber viroid, Peach latent mosaic viroid, Chrysan- themum chlorotic mottle viroid
Viroids are autonomously replicating, small singlestranded circular RNA molecules that do not code for proteins and may cause disease in infected, susceptible plants. Viroids have the ability to induce both RNA-mediated transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS) in infected plants. Viroid PTGS has also been demonstrated in a wheat germ extract system. A p...
متن کاملDigoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2008
ISSN: 1028-8880
DOI: 10.3923/pjbs.2008.1642.1645